Journal: BMC Microbiology

Article Title: Release of extracellular ATP by bacteria during growth

doi: 10.1186/1471-2180-13-301

Figure Lengend Snippet: ATP supplementation increases the stationary survival of bacteria. E. coli K12, Salmonella enterica Serovar Enteritidis (SE) or Salmonella enterica Serovar Typhimurium (ST) was cultured in M9 minimal medium or M9 minimal medium supplemented with 10 μM or 100 μM of ATP. The rate of survival was determined by comparing bacterial CFU/mL after 7 days of incubation to that after 1 day of incubation. The experiment was performed three times and results are from a representative experiment performed in triplicate. Error bars represent standard deviation. * p < 0.05, Student’s t -test.

Article Snippet: Culture media Luria Bertani (LB) broth and M9 minimal medium were from BD Diagnostics (Sparks, MD).

Techniques: Bacteria, Cell Culture, Incubation, Standard Deviation

Journal: Antibiotics

Article Title: A Pilot Study in Humans on the Urinary Tract Excretion of the FimH Inhibitor 1-Deoxymannose

doi: 10.3390/antibiotics14050498

Figure Lengend Snippet: Utilization of 1-deoxymannose on the growth of Escherichia. coli. Changes in turbidity (600 nm) caused by the growth of Escherichia coli (NBRC 3301) in a M9 minimal medium containing 1% of each sugar (DM, Man, glucose).

Article Snippet: Escherichia coli (NBRC 3301) was shaken at 37 °C for 17 h using 2 mL of M9 minimal medium (DifcoTM M9 Minimal Salt supplemented with MgSO 4 and CaCl 2 , Becton, Dickinson and Company, Franklin Lakes, NJ, USA) containing 1% glucose to obtain a culture solution.

Techniques: